diff --git a/android_webview/browser/aw_browser_main_parts.cc b/android_webview/browser/aw_browser_main_parts.cc
index 028d911881896f..26957372e4b2b8 100644
--- a/android_webview/browser/aw_browser_main_parts.cc
+++ b/android_webview/browser/aw_browser_main_parts.cc
@@ -153,19 +153,6 @@ void AwBrowserMainParts::RegisterSyntheticTrials() {
   }
   AwMetricsServiceAccessor::RegisterSyntheticFieldTrial(
       metrics, kWebViewApkTypeTrial, apk_type_string);
-
-  // If isolated splits are enabled at build time, Monochrome and Trichrome will
-  // have a different bundle layout, so measure N+ even though isolated splits
-  // are only supported by Android in O+.
-  if (apk_type == ApkType::MONOCHROME &&
-      base::android::BuildInfo::GetInstance()->sdk_int() >=
-          base::android::SDK_VERSION_NOUGAT) {
-    static constexpr char kIsolatedSplitsTrial[] = "IsolatedSplitsSynthetic";
-    AwMetricsServiceAccessor::RegisterSyntheticFieldTrial(
-        metrics, kIsolatedSplitsTrial,
-        base::android::BundleUtils::IsolatedSplitsEnabled() ? "Enabled"
-                                                            : "Disabled");
-  }
 }
 
 int AwBrowserMainParts::PreMainMessageLoopRun() {
diff --git a/base/android/bundle_utils.cc b/base/android/bundle_utils.cc
index 745606d045a96f..0135fc53800a1f 100644
--- a/base/android/bundle_utils.cc
+++ b/base/android/bundle_utils.cc
@@ -69,11 +69,6 @@ bool BundleUtils::IsBundle() {
   return Java_BundleUtils_isBundleForNative(AttachCurrentThread());
 }
 
-// static
-bool BundleUtils::IsolatedSplitsEnabled() {
-  return Java_BundleUtils_isolatedSplitsEnabled(AttachCurrentThread());
-}
-
 // static
 void* BundleUtils::DlOpenModuleLibraryPartition(const std::string& library_name,
                                                 const std::string& partition,
diff --git a/base/android/bundle_utils.h b/base/android/bundle_utils.h
index 76593254e33f0e..2efb54b51349dc 100644
--- a/base/android/bundle_utils.h
+++ b/base/android/bundle_utils.h
@@ -18,9 +18,6 @@ class BASE_EXPORT BundleUtils {
   // Returns true if the current build is a bundle.
   static bool IsBundle();
 
-  // Returns true if isolated splits are enabled.
-  static bool IsolatedSplitsEnabled();
-
   // Helper function asking Java to resolve a library path. This is required for
   // resolving a module library made available via SplitCompat, rather than in
   // its eventual fully-installed state.
diff --git a/base/android/java/src/org/chromium/base/BundleUtils.java b/base/android/java/src/org/chromium/base/BundleUtils.java
index 3981fa37c78821..5f16497d405021 100644
--- a/base/android/java/src/org/chromium/base/BundleUtils.java
+++ b/base/android/java/src/org/chromium/base/BundleUtils.java
@@ -77,7 +77,6 @@ public static void setIsBundle(boolean isBundle) {
         sIsBundle = isBundle;
     }
 
-    @CalledByNative
     public static boolean isolatedSplitsEnabled() {
         return BuildConfig.ISOLATED_SPLITS_ENABLED;
     }
diff --git a/chrome/browser/chrome_browser_field_trials.cc b/chrome/browser/chrome_browser_field_trials.cc
index 17cf846fa0b4ea..9e3f48f2368989 100644
--- a/chrome/browser/chrome_browser_field_trials.cc
+++ b/chrome/browser/chrome_browser_field_trials.cc
@@ -176,18 +176,6 @@ void ChromeBrowserFieldTrials::RegisterSyntheticTrials() {
     ChromeMetricsServiceAccessor::RegisterSyntheticFieldTrial(
         kBackgroundThreadPoolTrial, group_name);
   }
-
-  // If isolated splits are enabled at build time, Monochrome and Trichrome will
-  // have a different bundle layout, so measure N+ even though isolated splits
-  // are only supported by Android in O+.
-  if (base::android::BuildInfo::GetInstance()->sdk_int() >=
-      base::android::SDK_VERSION_NOUGAT) {
-    static constexpr char kIsolatedSplitsTrial[] = "IsolatedSplitsSynthetic";
-    ChromeMetricsServiceAccessor::RegisterSyntheticFieldTrial(
-        kIsolatedSplitsTrial,
-        base::android::BundleUtils::IsolatedSplitsEnabled() ? "Enabled"
-                                                            : "Disabled");
-  }
 #endif  // defined(OS_ANDROID)
 }