diff --git a/src/rules/flake8_pytest_style/rules/unittest_assert.rs b/src/rules/flake8_pytest_style/rules/unittest_assert.rs
index 6a78897eef3be..cf935e476fcc2 100644
--- a/src/rules/flake8_pytest_style/rules/unittest_assert.rs
+++ b/src/rules/flake8_pytest_style/rules/unittest_assert.rs
@@ -265,12 +265,15 @@ impl UnittestAssert {
                     .get("expr")
                     .ok_or_else(|| anyhow!("Missing argument `expr`"))?;
                 let msg = args.get("msg").copied();
-                let bool = create_expr(ExprKind::Constant {
-                    value: Constant::Bool(matches!(self, UnittestAssert::True)),
-                    kind: None,
-                });
-                let expr = compare(expr, Cmpop::Is, &bool);
-                Ok(assert(&expr, msg))
+                if matches!(self, UnittestAssert::False) {
+                    let unary_expr = create_expr(ExprKind::UnaryOp {
+                        op: Unaryop::Not,
+                        operand: Box::new(create_expr(expr.node.clone())),
+                    });
+                    Ok(assert(&unary_expr, msg))
+                } else {
+                    Ok(assert(expr, msg))
+                }
             }
             UnittestAssert::Equal
             | UnittestAssert::Equals
diff --git a/src/rules/flake8_pytest_style/snapshots/ruff__rules__flake8_pytest_style__tests__PT009.snap b/src/rules/flake8_pytest_style/snapshots/ruff__rules__flake8_pytest_style__tests__PT009.snap
index 08efe04b88723..6a3ee05488cb7 100644
--- a/src/rules/flake8_pytest_style/snapshots/ruff__rules__flake8_pytest_style__tests__PT009.snap
+++ b/src/rules/flake8_pytest_style/snapshots/ruff__rules__flake8_pytest_style__tests__PT009.snap
@@ -13,7 +13,7 @@ expression: diagnostics
     column: 23
   fix:
     content:
-      - assert expr is True
+      - assert expr
     location:
       row: 11
       column: 8
@@ -32,7 +32,7 @@ expression: diagnostics
     column: 23
   fix:
     content:
-      - assert expr is True
+      - assert expr
     location:
       row: 12
       column: 8
@@ -51,7 +51,7 @@ expression: diagnostics
     column: 23
   fix:
     content:
-      - assert expr is True
+      - assert expr
     location:
       row: 13
       column: 8
@@ -70,7 +70,7 @@ expression: diagnostics
     column: 23
   fix:
     content:
-      - "assert expr is True, msg"
+      - "assert expr, msg"
     location:
       row: 14
       column: 8
@@ -89,7 +89,7 @@ expression: diagnostics
     column: 23
   fix:
     content:
-      - "assert expr is True, msg"
+      - "assert expr, msg"
     location:
       row: 15
       column: 8
@@ -152,7 +152,7 @@ expression: diagnostics
     column: 24
   fix:
     content:
-      - assert True is False
+      - assert not True
     location:
       row: 22
       column: 8