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I ran the r0.8 version on data previously analyzed with the r0.5 version. I systematically get a ~10x lower coverage across the entire genome for all my samples (from ~100X with r0.5 to ~10X with r0.8).
Because of this, I have difficulty detecting variants in regions with lower coverage.
Is there an explanation related to the r0.8 version ? The data is the same. Same BAM files.
Thank you !
The text was updated successfully, but these errors were encountered:
There's been a lot of parameter updates between r0.5 and r0.8. So a lot of reads with lower mapqs or baseqs would not make the depth. So this difference is expected.
I would say yes, our analysis shows higher accuracy at 10x with new version. One thing I would suggest is that if you working with a diploid genome then you expect 5x per haplotype with 10x. With that and the error-rate of the sequencing device, it becomes difficult to call variants accurately. We generally suggest between 15x-20x to get a set of variants that are better suitable for high-quality downstream analysis.
Hello,
I ran the r0.8 version on data previously analyzed with the r0.5 version. I systematically get a ~10x lower coverage across the entire genome for all my samples (from ~100X with r0.5 to ~10X with r0.8).
Because of this, I have difficulty detecting variants in regions with lower coverage.
Is there an explanation related to the r0.8 version ? The data is the same. Same BAM files.
Thank you !
The text was updated successfully, but these errors were encountered: