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not producing PEPPER_HP_OUTPUT_2.vcf.gz output for sample data pepper polishing (v.04) #177

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plnspineda opened this issue Jan 27, 2023 · 0 comments

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@plnspineda
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plnspineda commented Jan 27, 2023

Hi! I am trying to test pepper polishing with this tutorial, however, I am not getting a PEPPER_HP_OUTPUT_2.vcf.gz file but instead a PEPPER_SNP_OUPUT.vcf.gz file. As per the log, I think PEPPER_HP_OUTPUT_2.vcf.gz is essential to continue to 4_DeepVariant.

These are the log files:
1_pepper_snp.log
2_margin_haplotag.log
3_pepper_hp.log
4_DeepVariant.log

Error happens at 4_DeepVariant with the following prompt:

ValueError: Not found: Could not open /hpcfs/users/xxx/ont-polishing-case-study/output/PEPPER_HP_OUTPUT_2.vcf.gz

Can I please ask how to troubleshoot this? Thank you very much!

@plnspineda plnspineda changed the title not producing haplotyped vcf output for sample data (v.04) not producing PEPPER_HP_OUTPUT_2.vcf.gz output for sample data pepper polishing (v.04) Jan 27, 2023
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