Install Monero node or choose a public one.
Install and configure monero-wallet-rpc service. Add disable-rpc-login=1 to your monero-wallet-rpc configuration file (currently RPC auth is not supported in Mitra). See example.
Start monero-wallet-rpc. Create a wallet for your instance:
mitractl create-monero-wallet "mitra-wallet" "passw0rd"
Add blockchain configuration to blockchains array in your configuration file.