diff --git a/packages/vite/src/node/plugins/importAnalysisBuild.ts b/packages/vite/src/node/plugins/importAnalysisBuild.ts
index 16e51e287db3a7..07e6a5c39c444c 100644
--- a/packages/vite/src/node/plugins/importAnalysisBuild.ts
+++ b/packages/vite/src/node/plugins/importAnalysisBuild.ts
@@ -634,13 +634,22 @@ export function buildImportAnalysisPlugin(config: ResolvedConfig): Plugin {
             )
             .join(',')}]`
 
-          s.append(`\
+          const mapDepsCode = `\
 function __vite__mapDeps(indexes) {
   if (!__vite__mapDeps.viteFileDeps) {
     __vite__mapDeps.viteFileDeps = ${fileDepsCode}
   }
   return indexes.map((i) => __vite__mapDeps.viteFileDeps[i])
-}`)
+}\n`
+
+          // inject extra code before sourcemap comment
+          const mapFileCommentMatch =
+            convertSourceMap.mapFileCommentRegex.exec(code)
+          if (mapFileCommentMatch) {
+            s.appendRight(mapFileCommentMatch.index, mapDepsCode)
+          } else {
+            s.append(mapDepsCode)
+          }
 
           // there may still be markers due to inlined dynamic imports, remove
           // all the markers regardless
diff --git a/playground/js-sourcemap/__tests__/js-sourcemap.spec.ts b/playground/js-sourcemap/__tests__/js-sourcemap.spec.ts
index d4e6888ab5edd8..da1a517ca25beb 100644
--- a/playground/js-sourcemap/__tests__/js-sourcemap.spec.ts
+++ b/playground/js-sourcemap/__tests__/js-sourcemap.spec.ts
@@ -129,5 +129,10 @@ describe.runIf(isBuild)('build tests', () => {
         "version": 3,
       }
     `)
+    //
+    const js = findAssetFile(/after-preload-dynamic.*\.js$/)
+    expect(js.trim().split('\n').at(-1)).toMatch(
+      /^\/\/# sourceMappingURL=after-preload-dynamic.*\.js\.map$/,
+    )
   })
 })