Numbat error with custom reference

[cv55]

Hello, thanks for the great tool.
I am trying to use Numbat using the average of my cells as reference. I use the provided function aggregate_counts(..), but i keep getting the error:

Error in mutate():
! Problem while computing p = t.test.pval(...).
ℹ The error occurred in row 1.
Caused by error in t.test.default():
! data are essentially constant
Traceback: ...

This is the first few reference genes and the annotation file to run the aggregate_counts().

[teng-gao]

Hello, the error message you show might suggest that you're using another function called aggregate_counts from a different package. You can try numbat::aggregate_counts instead.

Your reference gene should be a count matrix not normalized rates. Please refer to numbat::count_mat_ref and numbat::annot_ref for examples.