Compare the results from eQuilibrator (component contribution) or group contribution methods with experimental results from nucleoside phosphorolysis

My "Farm of Ideas" is supposed to store ideas I would like to develop into scientific progress some day. This is the template for all ideas in this Farm.

Measuring the invisible: exploiting a two-substrate two-product equilibrium to determine the decomposition rate of one of the products

Generalized yields of coupled enzymatic reactions.

It is possible to predict the spectrum of e.g. a nucleobase in a nucleoside-nucleobase conversion.

Finding thermodynamic cycles in the metabolism

Create an open database for the thermodynamics of enzyme-catalyzed reactions (and other metabolism relevant thermodynamic data)

compare two-point UV/Vis spectroscopy read-out with spectral unmixing

When determining equilibrium constants of multi-substrate / multi-product reactions, their ratios affect the uncertainty of the result.

When is it favorable to do two-step or one-pot coupled enzyme reactions?