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tli14 committed Aug 21, 2022
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* The entire data generated in this study is too large to store on Github, some example data for _`Escherichia coli`_ are available [online](https://bcb.unl.edu/PanMAGs/) for testing MAG simulation, generating mixed MAG datasets, extracting and comparing core genes, and evaluating downstream analysis.
* [`Anaconda`](https://www.anaconda.com/) is used to create conda environment to run Python scripts, the required package [conda_list](https://github.com/tli14/PanMAGs/blob/main/conda_list) can be downloaded using ```conda create --name <env> --file conda_list```.
* Information about R packages needed to run R codes can be found in [R_packages](https://github.com/tli14/PanMAGs/blob/main/R_packages).
* The four supplementary tables for this manuscript can be found the folder [supplementary tables](https://github.com/tli14/PanMAGs/tree/main/supplementary%20tables).

## Scripts
* **Python_Shell_scripts**
### Python_Shell_scripts
- **Genome_Data_Collection: collect and analyze genome data.**
- [download_all_complete_genome_fasta.sh](https://github.com/tli14/PanMAGs/blob/main/Python_Shell_scripts/1.Genome_Data_Collection/download_all_complete_genome_fasta.sh): download complete bacteria genomes from assembly_summary.txt.
- [download_genus_contaminaton_genomes.sh](https://github.com/tli14/PanMAGs/blob/main/Python_Shell_scripts/1.Genome_Data_Collection/download_genus_contaminaton_genomes.sh): download bacteria genomes as contamination datasets.
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- [core_alignment_tree.sh](https://github.com/tli14/PanMAGs/blob/main/Python_Shell_scripts/8.Phylogenetic_analysis/core_alignment_tree.sh): build tree based on core gene alignment by using fasttree.
- [tree_compare.sh](https://github.com/tli14/PanMAGs/blob/main/Python_Shell_scripts/8.Phylogenetic_analysis/tree_compare.sh): Compare two phylogenetic trees by using ETE3 toolkit.<br>

### R_code
* These R codes were used to generate figures and supplementary figures in the manuscript. For example, the "[Fig2.AB.frag_data.R](https://github.com/tli14/PanMAGs/blob/main/R_code/Fig2.AB.frag_data.R)" was used to generate Figure 2.A and Figure 2.B in the manuscript. The input files for generating Figure 2 can be found in "[Fig2.frag_incomp](https://github.com/tli14/PanMAGs/tree/main/R_code/Fig2.frag_incomp)".

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