add a new tool BAKTA for genome annotation

tools/bakta/.shed.yml

@@ -0,0 +1,13 @@
+name: "bakta"
+owner: "iuc"
+long_description: |
+"Bakta is a tool for the rapid & standardized annotation of bacterial genomes and plasmids from both isolates and MAGs.
+It provides dbxref-rich and sORF-including annotations in machine-readable JSON & bioinformatics standard file formats for automatic downstream analysis."
+categories:
+- Sequence Analysis
+remote_repository_url: "https://github.com/mesocentre-clermont-auvergne/galaxy-tools/tree/master/tools/bakta"
+homepage_url: "https://github.com/oschwengers/bakta"
+type: unrestricted
+auto_tool_repositories:
+  name_template: "{{ tool_id }}"
+  description_template: "{{ tool_name }}: rapid and standardized annotation of bacterial genomes, MAGs and plasmids"

tools/bakta/macro.xml

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+<?xml version="1.0"?>
+<macros>
+  <token name="@TOOL_VERSION@">1.4.2</token>
+  <token name="@VERSION_SUFFIX@">0</token>
+  <token name="@PROFILE@">21.05</token>
+  <xml name="version_command">
+     <version_command><![CDATA[bakta --version]]></version_command>
+  </xml>
+  <xml name="edam">
+    <edam_topics>
+        <edam_topic>topic_3174</edam_topic>
+    </edam_topics>
+    </xml>
+  <xml name="xrefs">
+    <xrefs>
+      <xref type='bio.tools'>Bakta</xref>
+    </xrefs>
+  </xml>
+  <xml name="requirements">
+    <requirements>
+      <requirement type="package" version="@TOOL_VERSION@">bakta</requirement>
+    </requirements>
+  </xml>
+  <xml name="citations">
+      <citations>
+        <citation type="bibtex">
+          @article{mbs:/content/journal/mgen/10.1099/mgen.0.000685,
+   author = "Schwengers, Oliver and Jelonek, Lukas and Dieckmann, Marius Alfred and Beyvers, Sebastian and Blom, Jochen and Goesmann, Alexander",
+   title = "Bakta: rapid and standardized annotation of bacterial genomes via alignment-free sequence identification",
+   journal= "Microbial Genomics",
+   year = "2021",
+   volume = "7",
+   number = "11",
+   pages = "",
+   doi = "https://doi.org/10.1099/mgen.0.000685",
+   url = "https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000685",
+   publisher = "Microbiology Society",
+   issn = "2057-5858",
+   type = "Journal Article",
+   keywords = "whole-genome sequencing",
+   keywords = "bacteria",
+   keywords = "metagenome-assembled genomes",
+   keywords = "plasmids ",
+   keywords = "genome annotation",
+   eid = "000685",
+   abstract = "Command-line annotation software tools have continuously gained popularity compared to
+   centralized online services due to the worldwide increase of sequenced bacterial genomes.
+   However, results of existing command-line software pipelines heavily depend on taxon-specific
+   databases or sufficiently well annotated reference genomes. Here, we introduce Bakta, a new command-line
+   software tool for the robust, taxon-independent, thorough and, nonetheless, fast annotation of bacterial genomes.
+   Bakta conducts a comprehensive annotation workflow including the detection of small proteins taking into account
+   replicon metadata. The annotation of coding sequences is accelerated via an alignment-free sequence identification
+   approach that in addition facilitates the precise assignment of public database cross-references.
+   Annotation results are exported in GFF3 and International Nucleotide Sequence Database Collaboration (INSDC)-compliant flat files,
+   as well as comprehensive JSON files, facilitating automated downstream analysis.
+   We compared Bakta to other rapid contemporary command-line annotation software tools in both targeted and taxonomically broad benchmarks
+   including isolates and metagenomic-assembled genomes. We demonstrated that Bakta outperforms other tools in terms of functional annotations,
+   the assignment of functional categories and database cross-references, whilst providing comparable wall-clock runtimes.
+   Bakta is implemented in Python 3 and runs on MacOS and Linux systems.
+   It is freely available under a GPLv3 license at https://github.com/oschwengers/bakta.
+   An accompanying web version is available at https://bakta.computational.bio.",
+  }
+        </citation>
+      </citations>
+  </xml>
+</macros>

tools/bakta/test-data/NC_002127.1.fna

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+>NC_002127.1 Escherichia coli O157:H7 str. Sakai plasmid pOSAK1, complete sequence
+TTCTTCTGCGAGTTCGTGCAGCTTCTCACACATGGTGGCCTGCTCGTCAGCATCGAGTGCGTCCAGTTTT
+TCGAGCAGCGTCAGGCTCTGGCTTTTTATGAATCCCGCCATGTTGAGTGCAGTTTGCTGCTGCTTGTTCA
+TCTTTCTGTTTTCTCCGTTCTGTCTGTCATCTGCGTCGTGTGATTATATCGCGCACCACTTTTCGACCGT
+CTTACCGCCGGTATTCTGCCGACGGACATTTCAGTCAGACAACACTGTCACTGCCAAAAAACAGCAGTGC
+TTTGTTGGTAATTCGAACTTGCAGACAGGACAGGATGTGCAATTGTTATACCGCGCATACATGCACGCTA
+TTACAATTACCCTGGTCAGGGCTTCGCCCCGACACCCCATGTCAGATACGGAGCCATGTTTTATGACAAA
+ACGAAGTGGAAGTAATACGCGCAGGCGGGCTATCAGTCGCCCTGTTCGTCTGACGGCAGAAGAAGACCAG
+GAAATCAGAAAAAGGGCTGCTGAATGCGGCAAGACCGTTTCTGGTTTTTTACGGGCGGCAGCTCTCGGTA
+AGAAAGTTAACTCACTGACTGATGACCGGGTGCTGAAAGAAGTTATGCGACTGGGGGCGTTGCAGAAAAA
+ACTCTTTATCGACGGCAAGCGTGTCGGGGACAGAGAGTATGCGGAGGTGCTGATCGCTATTACGGAGTAT
+CACCGTGCCCTGTTATCCAGGCTTATGGCAGATTAGCTTCCCGGAGAGAAACTGTCGAAAACAGACGGTA
+TGAACGCCGTAAGCCCCCAAACCGATCGCCATTCACTTTCATGCATAGCTATGCAGTGAGCTGAAAGCGA
+TCCTGACGCATTTTTCCGGTTTACCCCGGGGAAAACATCTCTTTTTGCGGTGTCTGCGTCAGAATCGCGT
+TCAGCGCGTTTTGGCGGTGCGCGTAATGAGACGTTATGGTAAATGTCTTCTGGCTTGATATTATATTGGA
+ATGCCTTTTTTCAAAGCAAATGATGTGGCTTTGGATAGAAGGTTTACGTTGATCTTATCAAAGTTTTTTT
+TAAAGAACGAAGCCGAGAGCTCAGATAAATCATTATATTCATCAGTTTTCGTAACTTTGTTTAATGTGTA
+ACTTGAAAACTTCTCGCCATTAAATGACGTATAGACGTAACGATCTTTTTTTCCACCGTTAGGAATTATT
+AAATCAAAAAAAACATCACCCTTGCTTTTCTTTTTCTTCAAGTCGGATTCGATTTTTGAGAAAAATTCGC
+TCGGGCTATAAATATCAGTAGCATAGACAATAAATAAAGTTTTATCTTTATTTTTTATTGCTTCTATTTG
+ATATTTTTTATCTTTTTTCATAATTTCAACCTAGCTACGCCACCATCTATTAATTGGCAAACGGTATCGA
+TGATTGCGATTGATTCTAATTTGTTAATTGTCTTCGTGTCAGCTATTCCTGGTTTCATATGAAACAAACC
+ATGCCTGTTCTCATGCCAGTAAGTGTAGCATTCACACAAAACTTCCGCTATTTCACCATTTATAGTTTCT
+TGGTGTATTTCTCTGATTATATATTTGGGTTTGTTTTCAGTGAAGTATTCGCCAAGGTTCTTTGATGATG
+ATGGATTGCAAACATCATTAAGTATTTGATATATAAAGCCTTCTATGGCTCTTAATGCAGAGAAGCAGTA
+TGTTGAGTAATCTTCCATTTCGACATCTATTTTTTTCATTATTAGCGAGCATGATAGCTGTTTTTTGATA
+TCTTCATGGATTTTATCGATGCTTTTTGGTAGTTTGCTATGCAACTCGGACTCAATAGTTTCTTTTTTTA
+TGTCAACATTAAATTCTTTATTTTTTTGTTCGACAATCTCTTTCATGTTTAGTATTGAGCACATGAAATC
+GTTAATCAAACTCGCGATTTGAAGGTATTTTCCTTGGAATTGAATAGAGCCGCGCTTGTAAATTTTTGCC
+CTGACCCTGTCACCATTGCTGGTGGTCATAATATATTGGTGTTTACAATTAGGATCGTTATTATTATCTT
+CTGTTATTGTTATCCCCTCTTCAGAAAGAAATTCAAATAGATTTGCCCTGTCATCATCACTGAATTTTGG
+AATGGTGTATTCAAAGTTCTTTGTGTCTGAATACAAACAGTTTTCTTTTATAATCAAGGCGATTTCATCA
+AAGTAAGTGTTATTTTGCCCAGACGCTCTTCCGATAGTGGTATTTCCGCCTGATGGCATTAGTTTTATTA
+AGAAGTCTATTCCTTTATATGTGCCAGATATGTGAGTTTCTCTTTCGTTTTTTACATTAGAGGAATAGTT
+TGTGACGCCATTCTGCGTCAGAGCAGACTCAATCTTGTCAATATTGATATTTAGTGCTTTAAACGGGTTC
+TGTGCCATTGGGTCAATCCGTTGTTTTTTTTGAATATGTACAGATCTTGTTTTTTTGTCAACGGAATAGC
+TGTTCGTTGACTTGATAGACCGATTGATTCATCATCTCATAAATAAAGAAAAACCACCGCTACCAACGGT
+GGTTTTCTCAAGGTTCGCTGAGCTACCAACTCTTTGAACCAAGGTAACTGGCTTGGAGGAGCGCAGTCAC
+CAAAATCTGTTCTTTCAGTTTAGCCTTAACAGGTGCATAACTTCAAGACAAACTCCTCTAAATCAGTTAC
+CAATGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAA
+GGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAA
+CTGAGATACCAACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATC
+CGGTAAGTGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCGGGGGGAAACGCCTGGTATCTTTA
+TAGTCCTGTCGGGTTTCGCCACCTCTGGCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGC
+CTATGGAAAAACGCCTGCGGTGCTGGCTTCTTCCGGTGCTTTGCTTTTTGCTCACATGTTCTTTCCGGCT
+TTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGACACCGCTCGCCGCAGTCGAA
+CGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTTATGTGACATTTTCTCCTTACGCT
+CTGTTGTGCCGTTCGGCATCCTGCCCTGAGCGTTATATCTCTGTGCTATTTTCTACTTCAAAGCGTGTCT
+GTATGCTGTTCTGGAG

tools/bakta/test-data/TEST_1/TEST_1.embl

@@ -0,0 +1,137 @@
+ID   contig_1; ; circular; DNA; ; PRO; 3306 BP.
+XX
+AC   contig_1;
+XX
+DE   plasmid unnamed1, complete sequence
+XX
+OS   .
+OC   .
+XX
+CC   Annotated with Bakta
+CC   Software: v1.4.2
+CC   Database: v3.0
+CC   DOI: 10.1099/mgen.0.000685
+CC   URL: github.com/oschwengers/bakta
+CC   
+CC   ##Genome Annotation Summary:##
+CC   Annotation Date                :: 08/17/2022, 09:35:24
+CC   Annotation Pipeline            :: Bakta
+CC   Annotation Software version    ::  v1.4.2
+CC   Annotation Database version    ::  v3.0
+CC   CDSs                           ::     3
+CC   tRNAs                          ::     0
+CC   tmRNAs                         ::     0
+CC   rRNAs                          ::     0
+CC   ncRNAs                         ::     0
+CC   regulatory ncRNAs              ::     0
+CC   CRISPR Arrays                  ::     0
+CC   oriCs/oriVs                    ::     0
+CC   oriTs                          ::     0
+CC   gaps                           ::     0
+XX
+FH   Key             Location/Qualifiers
+FH
+FT   source          1..3306
+FT                   /mol_type="genomic DNA"
+FT                   /plasmid="unnamed1"
+FT   gene            <2..736
+FT                   /locus_tag="DOGAIA_00005"
+FT   CDS             <2..736
+FT                   /product="hypothetical protein"
+FT                   /locus_tag="DOGAIA_00005"
+FT                   /translation="SSASSCSFSHMVACSSASSASSFSSSVRLWLFMNPAMLSAVCCCL
+FT                   FIFLFSPFCLSSASCDYIAHHFSTVLPPVFCRRTFQSDNTVTAKKQQCFVGNSNLQTGQ
+FT                   DVQLLYRAYMHAITITLVRASPRHPMSDTEPCFMTKRSGSNTRRRAISRPVRLTAEEDQ
+FT                   EIRKRAAECGKTVSGFLRAAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDRE
+FT                   YAEVLIAITEYHRALLSRLMAD"
+FT                   /codon_start=1
+FT                   /transl_table=11
+FT                   /protein_id="gnl|Bakta|DOGAIA_00005"
+FT                   /inference="ab initio prediction:Prodigal:2.6"
+FT   gene            complement(971..1351)
+FT                   /locus_tag="DOGAIA_00010"
+FT   CDS             complement(971..1351)
+FT                   /product="hypothetical protein"
+FT                   /locus_tag="DOGAIA_00010"
+FT                   /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
+FT                   SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
+FT                   DKINVNLLSKATSFALKKGIPI"
+FT                   /codon_start=1
+FT                   /transl_table=11
+FT                   /protein_id="gnl|Bakta|DOGAIA_00010"
+FT                   /inference="ab initio prediction:Prodigal:2.6"
+FT   gene            complement(1348..2388)
+FT                   /locus_tag="DOGAIA_00015"
+FT   CDS             complement(1348..2388)
+FT                   /product="hypothetical protein"
+FT                   /locus_tag="DOGAIA_00015"
+FT                   /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYK
+FT                   GIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDDDR
+FT                   ANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQIAS
+FT                   LINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSL
+FT                   IMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREI
+FT                   HQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVCQLIDGG
+FT                   VARLKL"
+FT                   /codon_start=1
+FT                   /transl_table=11
+FT                   /protein_id="gnl|Bakta|DOGAIA_00015"
+FT                   /inference="ab initio prediction:Prodigal:2.6"
+XX
+SQ   Sequence 3306 BP; 797 A; 692 C; 743 G; 1074 T; 0 other;
+     ttcttctgcg agttcgtgca gcttctcaca catggtggcc tgctcgtcag catcgagtgc        60
+     gtccagtttt tcgagcagcg tcaggctctg gctttttatg aatcccgcca tgttgagtgc       120
+     agtttgctgc tgcttgttca tctttctgtt ttctccgttc tgtctgtcat ctgcgtcgtg       180
+     tgattatatc gcgcaccact tttcgaccgt cttaccgccg gtattctgcc gacggacatt       240
+     tcagtcagac aacactgtca ctgccaaaaa acagcagtgc tttgttggta attcgaactt       300
+     gcagacagga caggatgtgc aattgttata ccgcgcatac atgcacgcta ttacaattac       360
+     cctggtcagg gcttcgcccc gacaccccat gtcagatacg gagccatgtt ttatgacaaa       420
+     acgaagtgga agtaatacgc gcaggcgggc tatcagtcgc cctgttcgtc tgacggcaga       480
+     agaagaccag gaaatcagaa aaagggctgc tgaatgcggc aagaccgttt ctggtttttt       540
+     acgggcggca gctctcggta agaaagttaa ctcactgact gatgaccggg tgctgaaaga       600
+     agttatgcga ctgggggcgt tgcagaaaaa actctttatc gacggcaagc gtgtcgggga       660
+     cagagagtat gcggaggtgc tgatcgctat tacggagtat caccgtgccc tgttatccag       720
+     gcttatggca gattagcttc ccggagagaa actgtcgaaa acagacggta tgaacgccgt       780
+     aagcccccaa accgatcgcc attcactttc atgcatagct atgcagtgag ctgaaagcga       840
+     tcctgacgca tttttccggt ttaccccggg gaaaacatct ctttttgcgg tgtctgcgtc       900
+     agaatcgcgt tcagcgcgtt ttggcggtgc gcgtaatgag acgttatggt aaatgtcttc       960
+     tggcttgata ttatattgga atgccttttt tcaaagcaaa tgatgtggct ttggatagaa      1020
+     ggtttacgtt gatcttatca aagttttttt taaagaacga agccgagagc tcagataaat      1080
+     cattatattc atcagttttc gtaactttgt ttaatgtgta acttgaaaac ttctcgccat      1140
+     taaatgacgt atagacgtaa cgatcttttt ttccaccgtt aggaattatt aaatcaaaaa      1200
+     aaacatcacc cttgcttttc tttttcttca agtcggattc gatttttgag aaaaattcgc      1260
+     tcgggctata aatatcagta gcatagacaa taaataaagt tttatcttta ttttttattg      1320
+     cttctatttg atatttttta tcttttttca taatttcaac ctagctacgc caccatctat      1380
+     taattggcaa acggtatcga tgattgcgat tgattctaat ttgttaattg tcttcgtgtc      1440
+     agctattcct ggtttcatat gaaacaaacc atgcctgttc tcatgccagt aagtgtagca      1500
+     ttcacacaaa acttccgcta tttcaccatt tatagtttct tggtgtattt ctctgattat      1560
+     atatttgggt ttgttttcag tgaagtattc gccaaggttc tttgatgatg atggattgca      1620
+     aacatcatta agtatttgat atataaagcc ttctatggct cttaatgcag agaagcagta      1680
+     tgttgagtaa tcttccattt cgacatctat ttttttcatt attagcgagc atgatagctg      1740
+     ttttttgata tcttcatgga ttttatcgat gctttttggt agtttgctat gcaactcgga      1800
+     ctcaatagtt tcttttttta tgtcaacatt aaattcttta tttttttgtt cgacaatctc      1860
+     tttcatgttt agtattgagc acatgaaatc gttaatcaaa ctcgcgattt gaaggtattt      1920
+     tccttggaat tgaatagagc cgcgcttgta aatttttgcc ctgaccctgt caccattgct      1980
+     ggtggtcata atatattggt gtttacaatt aggatcgtta ttattatctt ctgttattgt      2040
+     tatcccctct tcagaaagaa attcaaatag atttgccctg tcatcatcac tgaattttgg      2100
+     aatggtgtat tcaaagttct ttgtgtctga atacaaacag ttttctttta taatcaaggc      2160
+     gatttcatca aagtaagtgt tattttgccc agacgctctt ccgatagtgg tatttccgcc      2220
+     tgatggcatt agttttatta agaagtctat tcctttatat gtgccagata tgtgagtttc      2280
+     tctttcgttt tttacattag aggaatagtt tgtgacgcca ttctgcgtca gagcagactc      2340
+     aatcttgtca atattgatat ttagtgcttt aaacgggttc tgtgccattg ggtcaatccg      2400
+     ttgttttttt tgaatatgta cagatcttgt ttttttgtca acggaatagc tgttcgttga      2460
+     cttgatagac cgattgattc atcatctcat aaataaagaa aaaccaccgc taccaacggt      2520
+     ggttttctca aggttcgctg agctaccaac tctttgaacc aaggtaactg gcttggagga      2580
+     gcgcagtcac caaaatctgt tctttcagtt tagccttaac aggtgcataa cttcaagaca      2640
+     aactcctcta aatcagttac caatggctgc tgccagtggc gataagtcgt gtcttaccgg      2700
+     gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc      2760
+     gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga      2820
+     gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagtgg      2880
+     cagggtcgga acaggagagc gcacgaggga gcttccgggg ggaaacgcct ggtatcttta      2940
+     tagtcctgtc gggtttcgcc acctctggct tgagcgtcga tttttgtgat gctcgtcagg      3000
+     ggggcggagc ctatggaaaa acgcctgcgg tgctggcttc ttccggtgct ttgctttttg      3060
+     ctcacatgtt ctttccggct ttatcccctg attctgtgga taaccgtatt accgcctttg      3120
+     agtgagctga caccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg      3180
+     aagcggaaga gcgccttatg tgacattttc tccttacgct ctgttgtgcc gttcggcatc      3240
+     ctgccctgag cgttatatct ctgtgctatt ttctacttca aagcgtgtct gtatgctgtt      3300
+     ctggag                                                                 3306
+//

tools/bakta/test-data/TEST_1/TEST_1.faa

@@ -0,0 +1,6 @@
+>DOGAIA_00005 hypothetical protein
+SSASSCSFSHMVACSSASSASSFSSSVRLWLFMNPAMLSAVCCCLFIFLFSPFCLSSASCDYIAHHFSTVLPPVFCRRTFQSDNTVTAKKQQCFVGNSNLQTGQDVQLLYRAYMHAITITLVRASPRHPMSDTEPCFMTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRAAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRLMAD
+>DOGAIA_00010 hypothetical protein
+MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKKSKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNFDKINVNLLSKATSFALKKGIPI
+>DOGAIA_00015 hypothetical protein
+MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYKGIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDDDRANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQIASLINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSLIMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREIHQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVCQLIDGGVARLKL

tools/bakta/test-data/TEST_1/TEST_1.ffn

@@ -0,0 +1,6 @@
+>DOGAIA_00005 hypothetical protein
+TCTTCTGCGAGTTCGTGCAGCTTCTCACACATGGTGGCCTGCTCGTCAGCATCGAGTGCGTCCAGTTTTTCGAGCAGCGTCAGGCTCTGGCTTTTTATGAATCCCGCCATGTTGAGTGCAGTTTGCTGCTGCTTGTTCATCTTTCTGTTTTCTCCGTTCTGTCTGTCATCTGCGTCGTGTGATTATATCGCGCACCACTTTTCGACCGTCTTACCGCCGGTATTCTGCCGACGGACATTTCAGTCAGACAACACTGTCACTGCCAAAAAACAGCAGTGCTTTGTTGGTAATTCGAACTTGCAGACAGGACAGGATGTGCAATTGTTATACCGCGCATACATGCACGCTATTACAATTACCCTGGTCAGGGCTTCGCCCCGACACCCCATGTCAGATACGGAGCCATGTTTTATGACAAAACGAAGTGGAAGTAATACGCGCAGGCGGGCTATCAGTCGCCCTGTTCGTCTGACGGCAGAAGAAGACCAGGAAATCAGAAAAAGGGCTGCTGAATGCGGCAAGACCGTTTCTGGTTTTTTACGGGCGGCAGCTCTCGGTAAGAAAGTTAACTCACTGACTGATGACCGGGTGCTGAAAGAAGTTATGCGACTGGGGGCGTTGCAGAAAAAACTCTTTATCGACGGCAAGCGTGTCGGGGACAGAGAGTATGCGGAGGTGCTGATCGCTATTACGGAGTATCACCGTGCCCTGTTATCCAGGCTTATGGCAGATTAG
+>DOGAIA_00010 hypothetical protein
+ATGAAAAAAGATAAAAAATATCAAATAGAAGCAATAAAAAATAAAGATAAAACTTTATTTATTGTCTATGCTACTGATATTTATAGCCCGAGCGAATTTTTCTCAAAAATCGAATCCGACTTGAAGAAAAAGAAAAGCAAGGGTGATGTTTTTTTTGATTTAATAATTCCTAACGGTGGAAAAAAAGATCGTTACGTCTATACGTCATTTAATGGCGAGAAGTTTTCAAGTTACACATTAAACAAAGTTACGAAAACTGATGAATATAATGATTTATCTGAGCTCTCGGCTTCGTTCTTTAAAAAAAACTTTGATAAGATCAACGTAAACCTTCTATCCAAAGCCACATCATTTGCTTTGAAAAAAGGCATTCCAATATAA
+>DOGAIA_00015 hypothetical protein
+ATGGCACAGAACCCGTTTAAAGCACTAAATATCAATATTGACAAGATTGAGTCTGCTCTGACGCAGAATGGCGTCACAAACTATTCCTCTAATGTAAAAAACGAAAGAGAAACTCACATATCTGGCACATATAAAGGAATAGACTTCTTAATAAAACTAATGCCATCAGGCGGAAATACCACTATCGGAAGAGCGTCTGGGCAAAATAACACTTACTTTGATGAAATCGCCTTGATTATAAAAGAAAACTGTTTGTATTCAGACACAAAGAACTTTGAATACACCATTCCAAAATTCAGTGATGATGACAGGGCAAATCTATTTGAATTTCTTTCTGAAGAGGGGATAACAATAACAGAAGATAATAATAACGATCCTAATTGTAAACACCAATATATTATGACCACCAGCAATGGTGACAGGGTCAGGGCAAAAATTTACAAGCGCGGCTCTATTCAATTCCAAGGAAAATACCTTCAAATCGCGAGTTTGATTAACGATTTCATGTGCTCAATACTAAACATGAAAGAGATTGTCGAACAAAAAAATAAAGAATTTAATGTTGACATAAAAAAAGAAACTATTGAGTCCGAGTTGCATAGCAAACTACCAAAAAGCATCGATAAAATCCATGAAGATATCAAAAAACAGCTATCATGCTCGCTAATAATGAAAAAAATAGATGTCGAAATGGAAGATTACTCAACATACTGCTTCTCTGCATTAAGAGCCATAGAAGGCTTTATATATCAAATACTTAATGATGTTTGCAATCCATCATCATCAAAGAACCTTGGCGAATACTTCACTGAAAACAAACCCAAATATATAATCAGAGAAATACACCAAGAAACTATAAATGGTGAAATAGCGGAAGTTTTGTGTGAATGCTACACTTACTGGCATGAGAACAGGCATGGTTTGTTTCATATGAAACCAGGAATAGCTGACACGAAGACAATTAACAAATTAGAATCAATCGCAATCATCGATACCGTTTGCCAATTAATAGATGGTGGCGTAGCTAGGTTGAAATTATGA